Abstract

Daim ntawv tshaj tawm no muab cov ncauj lus kom ntxaws txog kev txheeb xyuas cov tshuaj toxicological thiab genotoxicological profile ntawm Pectin - muab tau Acidic Oligosaccharides (PAOS), ib daim ntawv tshwj xeeb ntawm Hloov Citrus Pectin. Kev tshuaj xyuas yog ua raws li kev ntsuas kev nyab xeeb los ntawm Garthoff li al. hauvRegulatory Toxicology thiab Pharmacology (2010).¹Cov ntsiab lus tseem ceeb ntawm kev tsom xam suav nrog cov khoom siv physicochemical characterization thiab purity profile, kev txhais lus tseem ceeb ntawm kev sib txawv.

hauv vitropivhauv vivogenotoxicity cov ntaub ntawv, thiab cov txheej txheem elucidation ntawm ib hom - tshwj xeeb urothelial hyperplasia pom hauv cov qauv nas. Cov ntaub ntawv sib sau ua ke tsim kom muaj kev ruaj ntseg profile rau cov khoom muaj prebiotic tshiab no.

Khoom yam ntxwv thiab Purity Profile

Qhov kev npaj PAOS tau soj ntsuam hauv cov kev tshawb fawb txog kev nyab xeeb yog tsim los ntawm cov zaub mov -qib pectin ntawm ib txoj kev enzymatic tshwj xeeb. Cov txheej txheem siv poly(methoxyl- -}galacturonide) lyase (EC 4.2.2.10) thiab poly(1,4-alpha-}D-}galacturonide) glycanhydrolase (EC 3.2.1.15), ob qho tib si muab los ntawm non-pathogenic ntawm {{10} cov tshuaj lom neeg tsis zoo.Aspergillus niger.¹Cov khoom uas tau tshwm sim yog sib xyaw ntawm cov kab oligomers ntawm galacturonic acid, nrog cov ntsiab lus tseem ceeb xws li galacturonic acid cov ntsiab lus ntawm 62 g / 100g, qhov hnyav molecular feem ntau qis dua 3800 Da (94 g / 100g), thiab qib ntawm methylation ntawm 50.¹

Rau txhua yam khoom siv rau kev noj haus lossis kev tshawb fawb daim ntawv thov, purity profile yog qhov tseem ceeb tshaj plaws. Cov batch ntawm PAOS siv nyob rau hauv cov kev tshawb fawb tau soj ntsuam kom huv si rau cov kab mob thiab residues. Cov theem ntawmhloov citrus pectin hnyav hlauthiab lwm yam kab mob tau pom tias muaj qhov tshwj xeeb tsawg, ua raws li cov qauv kev cai lij choj uas cuam tshuam thiab Kev Ua Haujlwm Zoo Agricultural.¹Cov ntsiab lus ntawm cov kev tshawb pom no tau nthuav tawm hauv qab no.

Cov ntaub ntawv muab rho tawm los ntawm Garthoff li al., 2010¹

Qhov tsawg microgram-ib -kg qib rau cov hlau hnyav hnyav xws li cadmium, lead, thiab mercury underscore qhov huv ntawm cov khoom siv raw thiab cov txheej txheem tsim khoom.

Kev Ntsuam Xyuas Tseem Ceeb ntawm Cov Ntaub Ntawv Genotoxicity

Kev soj ntsuam genotoxicity ntawm PAOS tau txais txiaj ntsig uas qhia txog qhov tseem ceeb ntawm kev txiav txim siab ua tiav daim duab toxicological, tshwj xeeb tshaj yog kev sib raug zoo ntawmhauv vitrothiabhauv vivocov ntaub ntawv. PAOS tau lees paub tias tsis yog mutagenic hauv cov kab mob rov qab hloov pauv (Ames kuaj) hla tsib hom kab mob, nrog thiab tsis muaj kev ua haujlwm metabolic.¹

Txawm li cas los xij, lubhauv vitromammalian cell assays tau ua ntau cov txiaj ntsig. Qhov kev ntsuam xyuas nas lymphoma tau suav tias yog "equivocal" vim yog cov lus teb zoo ntawm cov tshuaj cytotoxic siab heev uas tsis tuaj yeem rov ua dua thiab tsis muaj koob meej meej - teb kev sib raug zoo.¹Ib yam li ntawd, hauv kev kuaj chromosomal aberration siv CHO hlwb, PAOS tau pib suav tias yog clastogenic nyob rau hauv kev kho mob tas li. Kev ua raws li- kev tshawb nrhiav tau pom tias qhov kev cuam tshuam no yog nyob ntawm cytotoxicity thiab hnyav siv (DMSO). Thaum PAOS raug tshem tawm hauv kab lis kev cai nruab nrab- lub tsheb muaj feem cuam tshuam rau lub cev ntau dua - qhov kev nce ntxiv hauv cov hlwb tsis zoo tau pom tsuas yog ntawm qhov siab tshaj plaws pom zoo (

5000 ug / ml) thiab tau txuam nrog cytotoxicity siab (MI ntawm 34%), ua rau cov kws sau ntawv txiav txim siab tias nws tsis cuam tshuam txog kev lom neeg.¹Cov kev tshawb pom no qhia qhov zoo

hauv vitroCov txiaj ntsig tau zoo li cov khoom qub uas tau tsav los ntawm cytotoxicity, ib qho kev paub tsis meej pem hauv cov kev ntsuam xyuas.

Qhov tseeb hauv Vivo Safety Profile

Cov ntaub ntawv tseem ceeb tshaj plaws ntawm lub cev rau kev ntsuas kev pheej hmoo genotoxic yog los ntawmhauv vivokev kawm. Ib qho kev sim micronucleus tau muab tso rau hauv 13-lub lim tiam kev tshawb fawb txog qhov ncauj mob toxicity nyob rau hauv nas, ib qho qauv tsim uas ua kom muaj kev cuam tshuam ntev rau cov tshuaj ntsuam xyuas thiab nws cov metabolites. Txoj kev tshawb no, uas kuj suav nrog ibhauv uteroCov theem raug, pom tau tias PAOS tsis cuam tshuam rau niam txiv kev noj qab haus huv, fertility, reproduction, los yog pup yam ntxwv.¹Qhov tseem ceeb tshaj plaws, kev soj ntsuam ntawm cov pob txha femoral hlwb los ntawm cov nas nyob rau hauv siab - pawg koob tshuaj (10% PAOS hauv kev noj zaub mov, kev noj ntau tshaj

7 g / kg bw / hnub) qhia tias tsis muaj qhov nce ntawm qhov nruab nrab ntawm micronucleated polychromatic erythrocytes piv rau kev tswj hwm.¹Tsis tas li ntawd, qhov piv ntawm polychromatic rau normochromatic erythrocytes tsis hloov, qhia tias tsis muaj cytotoxicity rau cov pob txha pob txha.¹Qhov no tsis zoo

hauv vivoCov txiaj ntsig tau muab pov thawj tseeb tias PAOS tsis muaj cov haujlwm genotoxic nyob rau hauv lub cev.

Mechanistic Elucidation ntawm Ib Hom -Tshwj xeeb Artifact

Cov sub- txoj kev tshawb fawb tsis ntev los no tau nthuav tawm cov kab mob urothelial hyperplasia hauv cov nas noj 10% PAOS. Tsis yog qhov qhia tau hais tias muaj kev cuam tshuam ncaj qha los ntawm oligosaccharides, qhov no tau xav tias yog qhov cuam tshuam thib ob. Ib qho kev tshawb fawb ntxiv tau tsim los ntsuas qhov kev xav tias qhov hyperplasia yog tshwm sim los ntawm kev nce ntxiv hauv cov zis sodium concentration thiab pH, ib qho mob uas paub tias yuav ua rau muaj kev cuam tshuam tshwj xeeb hauv nas.¹Txoj kev tshawb no suav nrog ib pab pawg tau noj 10% PAOS ntxiv rau cov kua qaub

NH4 Cl. Cov txiaj ntsig tsis sib xws: cov zis pH qis hauv pawg NH4 Cltag nrho tiv thaiv kev loj hlob ntawm urothelial hyperplasia.¹Qhov no tau lees paub cov txheej txheem thiab pom tau tias cov txiaj ntsig tsis yog los ntawm PAOS tab sis yog hom -cov khoom ntiag tug tshwj xeeb ntawm cov zis physiology, uas tsis suav tias cuam tshuam rau tib neeg.¹

Qhov kev nruj me ntsis tau pom hauv cov kev tshawb fawb no qhia txog kev cog lus ntawm kev tshawb fawb ntawm cov neeg tsim khoom xws li Zhejiang Gold Kropn Biotechnology Co., Ltd. Raws li lub teb chaws siab -tech enterprise koom tes nrog rau sab saum toj -tier universities, lub tuam txhab tseem ceeb tsis yog tsuas yog kev tsim khoom tab sis kuj muaj kev nkag siab tob txog kev tshawb fawb ntawm nws cov khoom. Lawv siv cov enzymatic hydrolysis siab heev nyob rau hauv ib tug 100,000-qib GMP- ntawv pov thawj qhov chaw ua kom paub tseeb tias cov khoom ntawm high-purityhloov citrus pectinhaum rau kev thov kev tshawb fawb thiab kev txhim kho daim ntawv thov. Txhawm rau thov kev qhia tshwj xeeb, daim ntawv pov thawj ntawm kev tshuaj xyuas, lossis sib tham txog kev sib koom tes tshawb fawb, thov hu rau wilson@zjgykp.com.

Cov ntsiab lus: Ib qho zoo - Cov khoom muaj txiaj ntsig rau Kev Tshawb Fawb thiab Kev Txhim Kho

Hloov Kho Citrus Pectin (PAOS) ​​yog cov khoom siv nrog kev soj ntsuam kom zoo thiab muaj kev nyab xeeb zoo. Cov ntaub ntawv qhia tau hais tias nws tsis yog -mutagenichauv vivo, thiab tej kev txhawj xeeb los ntawmhauv vitroKev soj ntsuam tau raug rationalized li artifacts ntawm cytotoxicity. Kev soj ntsuam ntawm urothelial hyperplasia nyob rau hauv nas tau raug piav qhia raws li ib hom kab mob - cov lus teb tshwj xeeb rau kev hloov pauv urinary chemical thiab tsis cuam tshuam rau tib neeg. Cov ntaub ntawv muaj purity siab thiab tsawg heev ntawm cov pa paug, nrog rau cov hlau hnyav, ua kom nws ua tau zoo - cov khoom muaj txiaj ntsig thiab txhim khu kev qha rau kev tshawb fawb thiab kev loj hlob ntxiv hauv kev noj zaub mov thiab kws tshuaj.

Tej hauj lwm hais

pectin kev nyab xeeb 1.pdf